Manilkara zapota L. (Sapotaceae) seeds have been reported to exhibit antibacterial activity. The present study was carried out to investigate phytochemical and antioxidant profile of seeds of Manilkara zapota L. The aim of this research is to explore the anti-anthritic potential of this selected plant material. Five successive dilution of crude extract of its seeds were subjected for in vitro anti-arthritic activity using inhibition of protein denaturation method. The successive methanol extract have shown potent anti-arthritic activity by inhibition of protein denaturation method with increase in concentration. All the concentrations prepared were paving dose dependent anti-arthritic activity.