Isolation, purification and identification of the ethanol extract of sea cucumber (Stichopus hermanii) originating from the area of ​​Seira Island, Ambon Indonesia has been done. Extraction was carried out by maceration of dried sea cucumbers with 96% ethanol (after cleaning, drying, smoked, and cut into small pieces). Ethanol extract was fractionated by column chromatography (SiO₂, CHCl₃ - MeOH = 10: 1 ~ 2: 1) gave 4 fractions (Fr. I ~ Fr. IV). GC-MS analysis for fraction III which resulting in -glucosidase enzyme inhibition of 131.52 ppm consisting of compounds fatty acids and monosacharides.