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VOL. 10, ISSUE 3 (2026)
Directed chemical mutagenesis for targeted disruption of nitrogen and carbon metabolism to enhance industrial biochemical production in microbial cell factories
Authors
Dr. Sujeet Kumar
Abstract
Directed chemical
mutagenesis remains a powerful and practical strategy for improving microbial
strains used in industrial biotechnology. This study investigates the impact of
targeted metabolic pathway disruption on biochemical production in microbial cell
factories, with particular emphasis on nitrogen and carbon metabolism. Chemical
mutagens, including L-methionine-DL-sulfoximine (MSO), were employed to inhibit
glutamine synthetase and perturb nitrogen assimilation, thereby redirecting
intracellular metabolic flux. A reconstructed triplicate dataset, aligned with
reported mean values for amino acids, protease, and organic acid production,
was used to perform quantitative statistical analysis in the absence of raw
experimental replicates. Descriptive statistics and one-way analysis of
variance (ANOVA) were applied to evaluate differences between control and
mutant groups. The results demonstrated substantial improvements in mutant
strains, with amino acid production increasing by 45.00%, protease activity by
37.50%, and organic acid production by 41.67%. ANOVA analysis confirmed that
these differences were highly significant (p < 0.001) across all product
categories. The findings indicate that disruption of nitrogen metabolism,
coupled with redistribution of carbon flux through central metabolic pathways,
plays a critical role in enhancing biochemical productivity. Furthermore, the
integration of chemical mutagenesis with systems biology approaches provides a
comprehensive framework for understanding metabolic reprogramming and
optimizing microbial performance. This study highlights the industrial
relevance of pathway-targeted mutagenesis as a viable approach for developing
high-yield microbial strains for sustainable biochemical production.
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Pages:1-6
How to cite this article:
Dr. Sujeet Kumar "Directed chemical mutagenesis for targeted disruption of nitrogen and carbon metabolism to enhance industrial biochemical production in microbial cell factories". International Journal of Chemical Science, Vol 10, Issue 3, 2026, Pages 1-6
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